Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Primers and associated annealing temperatures.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques: Sequencing, Methylation Sequencing

Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Expression status of SAMSN1. (A) A total of 8/11 GC cell lines had reduced SAMSN1 mRNA expression compared with the FHs 74 cell line. (B) Quantification of SAMSN1 mRNA expression in GC and adjacent normal tissues. The median level of SAMSN1 mRNA expression was reduced in GC tissues compared with corresponding normal adjacent tissues, a finding that was independent of tumor differentiation status. Lines in the boxes indicate the median values. The upper and lower borders of the boxes indicate the quartile 4 and quartile 1 lines, respectively. The highest and lowest values are represented by horizontal lines. SAMSN1, SAM domain, SH3 domain and nuclear localization signals 1; GC, gastric cancer; NS, not significant.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Immunohistochemical analysis. (A) Representative GC specimens with an increased, equivalent and reduced intensity of SAMSN1 protein staining in cancerous tissue compared with adjacent normal tissue. Magnification, ×100. The ratio of expression levels of SAMSN1 mRNA between GC and corresponding normal adjacent tissue is shown below the figures. (B) A direct correlation was observed between SAMSN1 protein and mRNA expression in GC tissue specimens using the χ2 test (P=0.005). GC, gastric cancer; SAMSN1, SAM domain, SH3 domain and nuclear localization signals 1; N, normal tissue; T, tumor tissue.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Association between expression level of SAMSN1 mRNA and clinicopathological parameters in 175 patients.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Prognostic impact of SAMSN1 mRNA expression in GC patients. (A) Low SAMSN1 expression was associated with shorter overall survival times in patients with GC. The table under the graph indicates number of patients at risk for each group. (B) Expression levels of SAMSN1 mRNA according to the Union for International Cancer Control stage. Lines in the boxes indicate the median values. The upper and lower borders of the boxes indicate quatile 4 and quartile 1 lines, respectively. The highest and lowest values are represented by horizontal lines. SAMSN1, SAM domain, SH3 domain and nuclear localization signals 1; GC, gastric cancer; NS, not significant.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques: Expressing, Control

Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic factors for overall survival in 175 patients.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques:

Journal: Oncology Letters

Article Title: Prognostic relevance of SAMSN1 expression in gastric cancer

doi: 10.3892/ol.2016.5233

Figure Lengend Snippet: Survival analyses according to Union for International Cancer Control stage. (A) Survival differences between the low and high SAMSN1 expression groups were more apparent in patients with stage II/III GC. (B) Low SAMSN1 expression was associated with shorter disease free survival times in patients with stage II/III GC. SAMSN1, SAM domain, SH3 domain and nuclear localization signals 1; GC, gastric cancer.

Article Snippet: Sections were incubated for 1 h at room temperature with a rabbit polyclonal antibody raised against SAMSN1 (catalog no., 13063-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) diluted 1:400 in antibody diluent (Dako, Glostrup, Denmark).

Techniques: Control, Expressing

Journal: medRxiv

Article Title: SAMSN1 causes sepsis immunosuppression by inducing macrophages to express coinhibitory molecules that causes T cell exhaustion via KEAP1-NRF2 signaling

doi: 10.1101/2024.10.03.24314746

Figure Lengend Snippet: (a) The flow chart shows that proteins bound to SAMSN1 are first precipitated by IP, and then the transcription factors that bind to SAMSN1 and change under LPS stimulation are analyzed by mass spectrometry. Among the transcription factors that are upregulated after LPS stimulation, JUNB, ZFP36, NFKIBZ and KEAP1 can be found in the public datasets for RNA-Seq data after knocking them out. (b) GSE216352 dataset shows the expression of co-inhibitory molecules CD48, CD86, and CEACAM1 in WT and KEAP1-KO RAW264.7 cells. (c) Real-time RT-PCR detection of CD48/CD86/CEACAM1 mRNA levels after WT or SAMSN1-KO cells were stimulated with LPS. (d) Real-time RT-PCR detection of CD48/CD86/CEACAM1 mRNA levels in WT cells stimulated with LPS after treatment with the KEAP1 inhibitor ML385 (5μM for 1h). (e) Real-time RT-PCR detection of CD48/CD86/CEACAM1 mRNA levels in SAMSN1-KO cells stimulated with LPS after treatment with the agonist KI696 (20μM KI696 for 24h). (f) IP-WB assay showed that the specific binding of SAMSN1 and KEAP1 increased when WT cells were stimulated with LPS. (g) IP-WB assay detected the binding of KEAP1 and NRF2 in LPS-stimulated WT or SAMSN1-KO cells. Significance in (b-e) was assessed by 2-tailed Welch’s t test .

Article Snippet: Primary antibodies: Anti-p-ZAP70 antibody (CST Cat. # 2717); anti-ZAP70 antibody (CST Cat. # 2705); anti-p-LCK antibody (CST Cat. #2751); anti-LCK antibody (CST Cat. # 2984); anti-Actin antibody (HUABIO); anti-Tubulin antibody (HUABIO); KEAP1; anti-SAMSN1 antibody (AtaGenix).

Techniques: Mass Spectrometry, RNA Sequencing Assay, Expressing, Quantitative RT-PCR, Binding Assay

Journal: medRxiv

Article Title: SAMSN1 causes sepsis immunosuppression by inducing macrophages to express coinhibitory molecules that causes T cell exhaustion via KEAP1-NRF2 signaling

doi: 10.1101/2024.10.03.24314746

Figure Lengend Snippet: Schematic diagram: In the resting state, KEAP1 and NRF2 form a transcription complex in the cytoplasm of monocyte-macrophages and are in an inhibitory state. When sepsis occurs, the number of monocyte-macrophages increases significantly, in which SAMSN1 is highly expressed. SAMSN1 binds to KEAP1, causing NRF2 to dissociate from the complex and translocate into the nucleus, promoting the transcription of co-inhibitory molecules CD48/CD86/CEACAM1. Through cell-cell contact, these co-inhibitory molecules bind to corresponding receptors 2B4/TIM4/CD152 on the surface of T cells, causing T cell inhibition and exhaustion. High expression of SAMSN1 also switches macrophages into a suppressive phenotype, manifested by decreased phagocytic ability and ability to clear bacteria in the blood. Blockade of SAMSN1 by gene knockout or monoclonal antibody keeps KEAP1-NRF2 in a complex state, preventing NRF2 from entering the nucleus and promoting the transcription of downstream co-inhibitory molecules, thereby failing to inhibit T cell activation. Blockade of SAMSN1 also switches macrophages to a pro-immune phenotype with enhanced phagocytic ability, resulting in reduced organ injuries and increased survival rate.

Article Snippet: Primary antibodies: Anti-p-ZAP70 antibody (CST Cat. # 2717); anti-ZAP70 antibody (CST Cat. # 2705); anti-p-LCK antibody (CST Cat. #2751); anti-LCK antibody (CST Cat. # 2984); anti-Actin antibody (HUABIO); anti-Tubulin antibody (HUABIO); KEAP1; anti-SAMSN1 antibody (AtaGenix).

Techniques: Inhibition, Expressing, Bacteria, Gene Knockout, Activation Assay

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: Expression of the SAMSN1 gene as Determined by Affymetrix and Arrystar Microarrays and Relevant Clinical Data.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: A, as determined by Affymetrix and Arrystar microarrays. The standardized expression of SAMSN1 in normal brain, low-grade glioma and high-grade glioma was 1.00 ± 0.10, 1.53 ± 1.04 and 2.05 ± 1.53, respectively; the difference between the normal brain group and the high-grade glioma group was statistically significant (p = 0.037). B, as determined by TMA. SAMSN1 expression in normal brain and WHO grade III - IV glioma was 1.69 ± 1.30, 5.11 ± 3.33, 5.66 ± 2.93, 5.60 ± 3.12, and 5.86 ± 3.02, respectively. The level of SAMSN1 expression was higher in each grade of glioma than was found in normal brains (p<0.01), whereas the differences among grades of glioma were not significant (p>0.05). In nuclear, SAMSN1 expression in normal brain and WHO grade I - IV glioma was 0.00 ± 0.00, 0.00± 0.00, 0.37 ± 1.03, 0.03± 0.16, and 0.06±0.38, respectively. Grade II glioma exhibited a pivotal elevation of nuclear SAMSN1 expression compared to all other groups (p<0.05) (p<0.05).

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: Sixty-nine genes (including SAMSN1) were filtered out and associated with the prognosis of high-grade glioma, which could then be classified into two groups (Group 1: 16, 17, 20, 21, 22; Group 2: 18 and 19). In group 1, all patients died at the end of the follow-up, and the average overall survival (OS) time was 13 months. By contrast in group 2, the patients were alive at the end of the follow-up, and the average OS was 72.5 months. The prognosis of the two groups was obviously quite different.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques:

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: Expression of 69 Genes Relevant to the Prognosis of High-Grade Glioma in Affymetrix Microarrays.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: 523 GBM cases with full data of both clinical and SAMSN1 gene expression was downloaded from the TCGA website. Kaplan-Meier estimates (log-rank test) were made and found SAMSN1 expression was significantly affect the prognosis of GBM in both PFS and OS (p<0.05).

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: The expression of SAMSN1 was significantly associated with PFS (A) and OS (B) of GBM.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: The expression of SAMSN1 was significantly associated with PFS (A) and OS (B) of primary GBM, and with PFS (C) and OS (D) of secondary GBM as well.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: Clinical Characteristics of GBM Stratified by SAMSN1 Expression in TMA.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: Univariate Survival Analysis of GBM Stratified by Clinical Factors as Determined by Kaplan-Meier estimate.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques:

Journal: PLoS ONE

Article Title: SAMSN1 Is Highly Expressed and Associated with a Poor Survival in Glioblastoma Multiforme

doi: 10.1371/journal.pone.0081905

Figure Lengend Snippet: Multivariate Survival Analysis of GBM with Cox Proportional Hazards Regression Models.

Article Snippet: Immunohistochemical staining using a polyclonal anti-SAMSN1 antibody (1:500, Abgent, San Diego, CA, USA) was performed by the avidin-biotin complex (ABC) method (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing